In this study, Sharklet tested the micropattern’s ability to limit cell migration.
Platelet adhesion and activation are key events in thrombus or clot formation on blood-contacting biomaterials. Thus understanding the complex interactions between biomaterial surface properties and platelets is important for developing vascular access devices that limit thromboembolic events. Medical-grade poly(urethanes) are frequently used in blood-contacting medical devices due to their desirable mechanical properties and high level of hemocompatibility. Moreover, it has been shown that sub-platelet-sized micropatterns reduce platelet adhesion. Based on this evidence, we hypothesized that bio-inspired, antifouling Sharklet™ (SK) microtopographies replicated in biomedical thermoplastic poly(urethane) (TPU) reduce both platelet adhesion and activation compared to smooth (SM) controls.
Ventilator-associated pneumonia (VAP) is a leading hospital acquired infection in intensive care units despite improved patient care practices and advancements in endotracheal tube (ETT) designs. The ETT provides a conduit for bacterial access to the lower respiratory tract and a substratum for biofilm formation, both of which lead to VAP. A novel microscopic ordered surface topography, the Sharklet micro-pattern, has been shown to decrease surface attachment of numerous microorganisms, and may provide an alternative strategy for VAP prevention if included on the surface of an ETT. To evaluate the feasibility of this micro-pattern for this application, the microbial range of performance was investigated in addition to biofilm studies with and without a mucin-rich medium to simulate the tracheal environment in vitro.